Table 1.
Specific primers used for real-time qPCR expression analyses, primers final concentration (nM), GenBank accession numbers, and sizes of the amplified products in base pairs (bp).
| Genes | Nucleotide sequence (5′ → 3′) | nM | Accession. no. | bp |
|---|---|---|---|---|
| cldnb (*) | F: CCACACCATCATCCGAGAC R: CTCATCTTTAGGAGGGCAGTTG |
200 |
MW731470.1 XM_030437103.1 |
151 |
| cldnk (*) | F: CTCTGGCTCTGGGTGTCCTC R: CTGATGATGGAGTGGGCAGT |
200 |
MW731472.1 XM_030408301.1 |
154 |
| cldn3 | F: TGAGGGTGAACTGAGGAACA R: TGGAAGACAAAGAGCCTACG |
200 |
KF861991.1 XM_030440595.1 |
115 |
| cldn5 | F: CTGCTGTGCTGCTCCTGTC R: GTTCTGCGTGGCTCTCTTG |
400 |
MW731471.1 XM_030418403.1 |
81 |
| cldn7b | F: TACGCTCACGACATCATCCA R: CCAACTACAGCCAGGAAAGC |
200 |
MW731473.1 XM_030439139.1 |
116 |
| cldn12 | F: CCAGTTTACATTGCCTTTGTTC R: ATCTGCATTCTCATTCCTGAA |
200 |
KF861992.1 XM_030393069.1 |
180 |
| cldn15 | F: AAACCCACTTTGTGATTGCA R: TGTTTGACCTTCCCCTTACAA |
200 |
KF861993.1 XM_030431412.1 |
154 |
| cldn24 | F: GGGATGCTGGGAATGCTG R: AAAGTCCTCTTGGTGCGAAA |
200 |
MW731474.1 XM_030432503.1 |
83 |
| cldn34 | F: CTCCGCAGTCTCAACACGT R: CACAGGTAGATCCAGGTGCA |
200 |
MW731475.1 XM_030409841.1 |
158 |
| ocln1 (*) | F: AGAAACAGGCAATGAACTCG R: GGTCGGCGTCAAACTCTCT |
200 |
KF861990.1 XM_030434673.1 |
104 |
| ocln2 (*) | F: TGTTGTTGTTTCGGAGAGAGC R: CGACGACTGTTCTTGTCAGC |
200 |
MW731476.1 XM_030433803.1 |
138 |
| ocln3 (*) | F: TCGCGTTGTTGATGCTAATA R: TGGTTGACGAACACTCCTGA |
200 |
JQ692876.1 XM_030403521.1 |
148 |
| actb | F: TCTTCCAGCCATCCTTCCTCG R: TGTTGGCATACAGGTCCTTACGG |
200 |
X89920.1 XM_030406939.1 |
108 |
| eef1a | F: AGAGGCTGTCCCTGGTGA R: TGATGACCTGAGCGTTGAAG |
200 |
AF184170.1 XM_030411990.1 |
137 |
(*) See Supplementary Material for further details (Supplementary Figures S1, S2).