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. 2021 Dec 20;17(12):e1009953. doi: 10.1371/journal.pgen.1009953

Fig 3. mTOR regulates RNA Pol III activity during CIS escape.

Fig 3

A. Nanodrop quantification of total RNA level in senescent LS174T cells two days after mTOR inhibition (left, n = 3) or in senescent MCF7 cells two days after TSC2 inhibition (right, n = 3). B. Senescent LS174T cells were treated with mTOR inhibitors, and the relative expression of the indicated RNA was analyzed by RT-QPCR after 7 days (n = 3). C. Analysis of the expression of the indicated RNAs 2 days after TSC2 depletion in MCF7 senescent cells by RT-QPCR (n = 3). D. Senescent LS174T and MCF7 cells were transfected with a control siRNA or a siRNA directed against Brf1 during 24 hr and then washed with PBS and stimulated with fresh media. Cell extracts were recovered 2 days after Brf1 depletion and the expression of the indicated RNAs was analyzed by RT-QPCR (LS174T: n = 3; MCF7: n = 4, Kolmogorov-Smirnov test, * = p<0.05). E. Analysis of Bip expression by western blot 7 days after BRF1 depletion in LS174T and MCF7 senescent cells (n = 3). F, G. Senescent LS174T and MCF7 cells were transfected with a control siRNA or a siRNA directed against Brf1 during 24 hr and then washed with PBS and stimulated with fresh media. Nine days after Brf1 inactivation, the number of emerging clones was analyzed (n = 4, Kolmogorov-Smirnov test, * = p<0.05).