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. 2021 Dec 20;17(12):e1009953. doi: 10.1371/journal.pgen.1009953

Fig 4. Regulation of tRNA synthesis during senescence and CIS escape.

Fig 4

A, B. Expression of the indicated RNAs in growing or senescent cells (LS174T n = 4, MCF7 n = 4, Kolmogorov-Smirnov test, * = p<0.05). C. ChIP analysis of the binding of the type III RNA polymerase (POLR3A) in senescent or growing LS174T cells (n = 3). D. LS174T and MCF7 were transfected with a control siRNA or a siRNA directed against Maf1 48h prior to chemotherapeutic treatment. After senescence induction, cells were washed with PBS and stimulated with fresh media. Ten days later, the number of emerging clones was analyzed (n = 4, Kolmogorov-Smirnov test, * = p<0.05). E. Analysis of the expression of the indicated RNAs in senescent or emergent cells (after 7 days, LS174T n = 5, MCF7, n = 4, Kolmogorov-Smirnov test, * = p<0.05, ** = p<0.01).