Skip to main content
. 2021 Dec 20;17(12):e1009953. doi: 10.1371/journal.pgen.1009953

Fig 6. The LARS and YARS aminoacyl-tRNA synthetases are necessary for CIS escape.

Fig 6

A. Senescent LS174T and MCF7 cells were transfected with a control siRNA or a siRNA directed against Maf1 during 24 hr and then washed with PBS and stimulated with fresh media. Cell extracts were recovered 2 days after Maf1 depletion and the expression of the indicated RNAs was analyzed by RT-QPCR (LS174T: n = 3; MCF7: n = 4, Kolmogorov-Smirnov test, * = p<0.05). B. Cells were treated and transfected as described above. Nine days after Maf1 inactivation, the number of emerging clones was analyzed (n = 4, Kolmogorov-Smirnov test, * = p<0.05). C. LS174T and MCF7 senescent cells were transduced with an empty vector (pLKO.1) or a vector expressing the tRNA-Tyr-GTA or the tRNA-Leu-CAA. Ten days after, the number of emerging clones was analyzed (n = 4). D. Quantitative proteomic analysis of proteins involved in tRNA biogenesis between growing and LS174T senescent cells (n = 3). E. Senescent LS174T and MCF7 cells were transfected with a control siRNA or a siRNA directed against LARS, YARS or CARS. The depletion of the specifics Aminoacyl-tRNA Synthetases was validated by western blot 2 days after the transfection (n = 2). F. Senescent LS174T and MCF7 cells were transfected with a control siRNA or a siRNA directed against LARS, YARS or CARS for 24 hr. The number of emergent clones was evaluated 9 days later (LS174T n = 6, MCF7 n = 5, Kolmogorov-Smirnov test, ** = p<0.01).