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. 2021 Dec 20;17(12):e1009953. doi: 10.1371/journal.pgen.1009953

Fig 7. YARS and LARS regulate E2F targets during CIS escape.

Fig 7

A. Senescent cells were transfected with a control siRNA or a siRNA directed against YARS. Cell extracts were analyzed by SWATH quantitative proteomics and GSEA analysis 2 days after depletion (n = 3). B. Analysis by RT-QPCR and western blot of E2F1 proliferative targets in MCF7 cells following YARS depletion (n = 4, Kolmogorov-Smirnov test, * = p<0.05, n = 2 for western blot). C. Senescent cells were transfected with a control siRNA or a siRNA directed against YARS or LARS. After two days, FACS analysis was performed to analyze the cell cycle profile of the indicated cells (n = 5, Kolmogorov-Smirnov test, ** = p<0.01). D. Senescent MCF7 cells were transfected as above, FACS analysis was performed to analyze apoptotic cells (n = 5, Kolmogorov-Smirnov test, ** = p<0.01). The expression of the indicated mRNAs was analyzed in parallel by RT-QPCR (n = 4, Kolmogorov-Smirnov test, * = p<0.05). E. MCF7 or LS174T senescent cells were transfected with a control siRNA or a siRNA directed against LARS. Cell extracts were analyzed by SWATH quantitative proteomics and GSEA analysis 2 days after depletion (n = 3). In parallel, the indicated mRNAs were analyzed by RT-QPCR in MCF7 cells following LARS depletion (n = 4, Kolmogorov-Smirnov test, * = p<0.05). F. LS174T senescent cells were transfected as above, FACS analysis was performed to analyze apoptotic cells (n = 4). The expression of the indicated mRNAs was analyzed in parallel by RT-QPCR (n = 3).