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. 2021 Dec 20;8:780621. doi: 10.3389/fmolb.2021.780621

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Copyright © 2021 Lu, Su, Wang, Zhang, Ma, Li and Qiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dysregulation of selected tsRNAs in NPC by qRT-PCR. We selected 10 tsRNAs dysregulated in NPC for custom qRT-PCR. (A) Expression of 5 tsRNAs upregulated in NPC. (B) Expression of 5 tsRNAs downregulated in NPC as well. All data were analyzed using Student’s t-test (*p < 0.05, **p < 0.01). ROC curve analysis of (C) tRF-1:28-Val-CAC-2, (D) tRF-1:24-Ser-CGA-1-M3, and (E) tRF-55:76-Arg-ACG-1-M2. (F) Examples of tRNA or pre-tRNA structures of the three validated tsRNAs were depicted. The red nucleotides indicate the presence of the three validated tsRNA sequences.