Figure 1.

EPO reduces antibody formation after NP-KLH immunization and heart transplantation in mice. (A) Experimental design: B6 mice were immunized with NP-KLH on day 0 and treated with EPO (10,000 IU/kg, i.p.) or vehicle control starting from day 0 to day 10, when the animals were euthanized. (B) Total and (C) high affinity anti-NP antibodies were quantified by ELISA using BSA with different degrees of NP conjugation. (D) B6 recipients of an allogeneic heterotopic transplantation from BALB/c mice were treated with EPO (10,000 IU/kg, i.p.) or vehicle control from the day of transplant up to day 14 post-transplant, when the animals were euthanized. (E)–(G) Representative plots of donor-specific antibodies (DSA) IgG (E), MFI levels (F), and titers (G) in EPO- and vehicle-treated mice. Data represent median±interquartile range (IQR). Empty circles represent male animals, full circles females. **P<0.01 between EPO and vehicle. Data were analyzed using unpaired Mann–Whitney test.