Figure 4.

EPO reduces anti-sheep red blood cell (SRBC) antibody production in mice lacking EPOR on B cells. (A) EPOR^fl/fl CD19-Cre^POS and CD19-Cre^NEG mice were immunized with SRBC on day 0 and 6 and treated with EPO (10,000 IU/kg i.p.) or vehicle control starting from day -1 to day 12. At day 12, we euthanized the mice to measure GC B cells, T_FH, and T_FR cells. Representative plots (B) and data quantification (C) for B220⁺Fas⁺GL7⁺ GC B cells. Representative plots (D) and data quantification for CD4⁺PD1⁺CXCR5⁺Foxp3^- T_FH (E), CD4⁺PD1⁺CXCR5⁺Foxp3⁺ T_FR (F), and T_FH/T_FR ratio (G) in the same mice at the time of sacrifice. Data represent median±IQR. Empty circles represent male animals, full circles represent females. *P<0.05; **P<0.01. Data were analyzed using unpaired Mann–Whitney test.