Figure 5.

EPO does not affect anti-SRBC antibody production in mice lacking EPOR on T cells. (A) EPOR^fl/fl CD4-Cre^POS and CD4-Cre^NEG mice were immunized with SRBC on day 0 and 6 and treated with EPO (10,000 IU/kg i.p.) or vehicle control starting from day -1 to day 12. At day 12, we euthanized the mice to measure T_FH, T_FR, GC, and class switched B cells. (B)–(G) Representative plots (B), (E) and data quantification for GC (C), (D) and class switched (F), (G) B cells. (H)–(M) Representative plots (H) and data quantification for CD4⁺PD1⁺CXCR5⁺Foxp3^- T_FH (I), (J), CD4⁺PD1⁺CXCR5⁺Foxp3⁺ T_FR (K), (L), and T_FH/T_FR ratio (M). Data represent median±IQR. Empty circles represent male animals, full circles represent females. *P<0.05; **P<0.01. Data were analyzed using unpaired Mann–Whitney test.