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. 2021 Oct;32(10):2561–2578. doi: 10.1681/ASN.2021010133

Figure 9.

Figure 9.

miR-23b regulates the BMP pathway through Grem2. (A) Luciferase activity in HEK 293A cells transfected with GREM2, TFRC, and JARID2 3′-UTR reporter constructs demonstrated binding of miR-23b to the 3′-UTR of each reporter. (B) Western blot analysis for Grem2 and BMP pathway related proteins in kidneys of 3- and 5-month-old WT and miR-23b−/− mice. (C) Western blot analysis of BMP pathway–related proteins and TFRC in HMC driven to overexpress or downregulate Grem2 and miR-23b. (D) Western blot analysis of SMAD4, Grem2, and TFRC in HMC driven to overexpress SMAD4; RT-qPCR quantification of BMP2 (E) and miR-23b (F) expression in HMC treated with Grem2 siRNA and protein, miR-23b agomir, and antagomir, miR-23b agomir combined with Grem2 siRNA or protein, SMAD4-activated plasmid, SMAD4-activated plasmid combined with miR-23b agomir, and miRNA or siRNA-negative control. (G) A schematic model of potential regulation and function of miR-23b. *P<0.05, **P<0.01, ***P<0.001. Data are shown as the mean±SEM.