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. 2021 Sep 14;20:15330338211038492. doi: 10.1177/15330338211038492

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — NANOG promotes cancer cell characteristics in ESCC by activating IL-6/STAT3 signaling. (A and B) Expression of IL-6/STAT3, etc was detected by western blotting (A. overexpression and B. knockdown). (C) qPCR tests were used to determine the mRNA expression of NANOG and IL-6 for ectopic expression of different doses of NANOG. (D) Comparison of p-JAK2, total STAT3, p-STAT3, and IL-6 expression by immunoblotting for ectopic expression of different NANOG doses in Eca-109 cells. (E) The level of IL-6 secreted into the growth medium upon ectopic NANOG expression (lower panel) was detected using an ELISA kit, and compared with that of standard samples (upper panel) (*P < .05; **P < .01; ***P < .001).

Abbreviations: ESCC, esophageal squamous cell carcinoma; STAT3, signal transducer and activator of transcription 3; IL-6, interleukin 6; qPCR, quantitative real-time PCR; ELISA, enzyme-linked immunosorbent assay; p-JAK2, phospho-Janus kinase 2.