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. Author manuscript; available in PMC: 2022 Apr 15.
Published in final edited form as: Cancer Res. 2021 Sep 21;81(20):5325–5335. doi: 10.1158/0008-5472.CAN-21-1545

Figure 2: ARID1A directly represses the XBP1 gene.

Figure 2:

(A) ARID1A ChIP-seq and input tracks in the XBP1 gene locus in ARID1A wildtype RMG1 cells (30). (B) RNA polymerase II ChIP-seq and input tracks in the XBP1 gene locus in control and ARID1A knockout RMG1 cells (21). (C) The association of ARID1A and SNF5 with the XBP1 gene promoter in control and ARID1A knockout RMG1 cells was determined by ChIP-qPCR analysis. An isotype matched IgG was used as a negative control. n = 3 biologically independent experiments. (D) Expression of FLAG, ARID1A and a loading control β-actin in parental control and endogenous ARID1A FLAG tagged RMG1 cells was determined by immunoblot. (E) The association of ARID1A with the XBP1 gene promoter in control parental and FLAG-tagged endogenous ARID1A-expressing cells was determined by ChIP-qPCR analysis. An isotype matched IgG was used as a negative control. n = 3 biologically independent experiments. P values were calculated using two-tailed Student’s t-test. Error bars represent mean with SD.