Table 4.
Antigenicity, allergenicity, solubility, and physicochemical property assessments of the primary sequence of NRM-based vaccine construct, the translated peptide form of mRNA vaccine
| Sl. No | Features | Assessment |
|---|---|---|
| 1 | Antigenicity | 0.6661 (Probable ANTIGEN) by VaxiJen v2.0 |
| 2 | Allergenicity | Probable non-allergen (AllerTOP v.2.0) |
| 3 | Solubility | 0.850 (Soluble) |
| 4 | Number of amino acids | 196 |
| 5 | Molecular weight | 21368.68 Dalton |
| 6 | Theoretical isoelectric point (pI) | 10.23 |
| 7 | Total number of atoms | 3056 |
| 8 | Formula | C959H1548N272O274S3 |
| 9 | Estimated half-life |
4.4 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
| 10 | Instability index | 26.23 (Stable) |
| 11 | Aliphatic index | 78.01 |
| 12 | Grand average of hydropathicity (GRAVY) | − 0.470 |