Figure 2.

Workflow schematic of the study. Experimental mice (N=30) were injected with single-cell suspension of patient SUTI151 and primary tumors were allowed to grow. Age matched control mice (N=5) were injected with PBS. At six weeks post-implant, seven mice with intact primary tumors were euthanized (Group A). The remaining mice (N=12) received tumorectomies and were euthanized at 13–14 weeks post-implant (Group B) and 15–16 weeks post implant (Group C) respectively. For each cohort, at the time of euthanasia, blood was drawn for CTC immunostaining and enumeration, while organs were collected for H&E analysis of metastases.