TABLE 3.
exo1-dependent mutator genes and mutations identifieda
| Mutant strainb | Mutator phenotype ratingc | EDM gene | Nucleotide change | Amino acid change |
|---|---|---|---|---|
| RKY4170 (H9) | 5 | MLH1 | G56A | G19D |
| RKY4171 (H3) | 4 | MLH1 | G82A | A28T |
| RKY4172 (C5) | 5 | MLH1 | G121A | A41T |
| RKY4173 (E15) | 4 | MLH1 | C470T | P157L |
| RKY4174 (C15) | 4 | MLH1 | C680T | T227I |
| RKY4175 (H2) | 5 | MLH1 | G794A | R265K |
| RKY4176 (J2) | 5 | MLH1 | G1640A | R547K |
| RKY4177 (D11) | 5 | PMS1 | C389T | A130V |
| RKY4178 (C14) | 4 | PMS1 | G479A | G160D |
| RKY4179 (F13) | 3 | PMS1 | G913A | G305S |
| RKY4180 (E13) | 3 | PMS1 | C2246T | T749I |
| RKY4181 (C11) | 4 | PMS1 | G2320A | D774N |
| RKY4182 (C2)d | 5 | PMS1 | G2701A | D901N |
| RKY4183 (B9) | 4 | MSH2 | C2285T | S762F |
| RKY4184 (J1) | 2 | MSH2 | G1623A | M541I |
| RKY4185 (D15) | 2 | MSH3 | G2470A | G824R |
| RKY4186 (J10) | 3 | POL30 | G427A | E143S |
| RKY4188 (J3) | 3 | RNR1 | G811T | G271S |
| RKY4187 (I1) | 3 | POL32 | C136T | Q46STP |
a
The EDM genes were identified by complementation analysis, and then the sequence of each relevant gene was determined. The base and amino acid changes given are numbered assuming that the A of the initiating ATG of each gene is numbered 1.
b
See Table 2, footnote a.
c
The numbers are a qualitative rating of the mutator phenotype of each exo1Δ edmx combination in patch tests like those shown in Fig. 1: 5, complete defect like that seen in an msh2Δ mutant; 4, 75% defect; 3, 50% defect; 2, 25% defect; 1, ∼1% defect like that seen in an exo1Δ mutant; −, wild type.
d
The phenotype of this mutant was partially suppressed only in the presence of a 2μm plasmid containing EXO1.