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AC16 cells were treated with 3.125 and 6.25 μM of GA, MTS, MTS-H3R6, MTS-H3R9, GA-MTS-H3R6, and GA-MTS-H3R9 for 16 h and were subsequently incubated with 5 μl of PI for 20 min. (A) PI internalization using flow cytometry analysis. (B) Quantitative graph showing each peptide from the PI fluorescence data shown in panel (A). GA, gallic acid; MTS, mitochondria targeting sequence; PI, propidium iodide.
