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Detection of DmRFC4 by immunofluorescence in interphase Drosophila cultured cells. Dmel2 cells were incubated in BrdU for 5 or 45 min and processed for immunofluorescence detection of RFC4 and incorporated BrdU. All replicating cells show homogeneous nuclear staining for RFC4, which is not restricted to the sites of active replication (5-min BrdU incubation). Scale bar = 10 μm.
