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. 2021 Dec 21;12:789647. doi: 10.3389/fimmu.2021.789647

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Copyright © 2021 Lai, Zhang, Jiang, Mou, Li, Xi, Wu, Gao, Zhang, Chen, Huang, Li, Cai, Li, Zheng and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRANK1 overexpression decreases dendritic spine density in rat primary cortical neurons. (A) Representative microphotographs of dendrites from neurons transfected with either control or TRANK1 overexpression vectors plus the Venus vector (which encodes EGFP protein) at DIV14. Scale bar: 5 µm. (B) Density of total, stubby-shaped, thin-shaped, and mushroom-shaped spines in neurons overexpressing TRANK1 compared with control neurons. Dendritic spine density was quantified as the number of spines normalized to 10 μm of dendritic length (**p < 0.001, *p < 0.01, n=15/16 neurons per condition). ns, not significant.