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. 2021 Dec 21;9:723804. doi: 10.3389/fcell.2021.723804

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Copyright © 2021 Xue, Wang, Wang, Lyu, Mo, Fan, Li, Yen, Yu, Liu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The elmo1 was cell-autonomously required for the motility of leukocytes in zebrafish larvae. (A) Track path of neutrophils expressing lyz:GFP (control) or lyz:elmo1^ze-GFP (lyz:elmo1^ze) on the yolk sac in the elmo1^−/− and sibling larvae recorded by live imaging at 3 dpf. Each line represents the migration path of a single cell recorded by live imaging. Scale bar: 50 μm. (B) Quantification of the neutrophils migration speed of the control and elmo1ze group in elmo1^−/− (14 cells of 4 larvae, 17 cells of 6 larvae) and sibling (11 cells of 4 larvae, 17 cells of 6 larvae) larvae. Compared with the control group, the migration speed of neutrophils expressing lyz:elmo1^ze was significantly increased in the elmo1^−/− larvae. Each dot represents the average speed of one individual cell. Three independent experiments were performed. Here present the summarized results of three experiments. One-way ANOVA, ns: no significance, ****p < 0.001. (C) Track path of T-cells expressing lck:DsRedx (control) or lck:elmo1^ze-GFP (lck:elmo1^ze) within the thymus in elmo1^−/− and sibling larvae recorded by live imaging at 5 dpf. Each line represents the migration path of a single cell recorded by live imaging. Scale bar: 10 μm. (D) Quantification of the T-cells migration speed of control and elmo1^ze group in elmo1^−/− (15 cells of 4 larvae, 30 cells of 9 larvae) and sibling (22 cells of 4 larvae, 31 cells of 9 larvae) larvae. Compared with the control group, the migration speed of T-cells expressing lck:elmo1^ze was significantly increased in the elmo1^−/− larvae. Each dot represents the average speed of one individual cell. Three independent experiments were performed. Here present the summarized results of three experiments. One-way ANOVA, ns: no significance, ****p < 0.001.