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. 2021 Mar 23;43(1):111–120. doi: 10.1038/s41401-021-00628-1

Fig. 1. Effect of aprotinin on urinary protease activity and ENaC-mediated sodium transport.

Fig. 1

a Urinary aprotinin concentration after implantation of aprotinin-containing pellets with sustained release (2 mg/day over 10 days) in mice treated with control or low-salt diet. Note that there is a peak soon after implantation that can be explained by rapid release of unbound aprotinin. Data are only descriptive (each n = 2–4). b Urinary protease activity given in relative fluorescence units (RFU) in mice under control or low-salt diet with or without aprotinin treatment (each n = 6). c, d Natriuretic response to vehicle (Veh.) (5 mL/kg  bw) and amiloride (Amil.) (10 mg/kg  bw) under control or low-salt diet with or without aprotinin treatment (c). The calculated ratio corresponding to the slope in c indicating amiloride-sensitive natriuresis (d) (each n = 6). Data and statistical analysis: arithmetic means ± SEM. b, d One-way ANOVA followed by the Dunnett’s test; c One-way ANOVA followed by the Sidak’s test or Kruskal–Wallis test followed by the Dunn’s test. *P< 0.05, aprotinin vs. placebo treatment, §P < 0.05, low-salt vs. control diet, #P < 0.05 amiloride vs. vehicle treatment.