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. 2021 Mar 25;43(1):229–239. doi: 10.1038/s41401-021-00632-5

Fig. 4. Cross-reactivity analysis of Cpd39 and the inhibitory effect of Cpd39 on AR or AR-V7 regulated genes in PCa cells.

Fig. 4

a–c Cross-reactivity analysis of Cpd39 inhibition. d Effect of compounds on AR nuclear localization. Microscopic images of LNCaP cells treated with 10 μM compounds and 5 nM DHT or DMSO only (magnification ×600; scale bar = 20 μm) (n = 3). e The mRNA expression levels of AR target genes including KLK3 (PSA), TMPRSS2, ACPP, and FKBP5 analyzed by quantitative analysis after LNCaP cells treated with 10 µM compounds and 10 nM DHT. *P < 0.05 versus DHT group (n = 3 in each group). f Protein expression of AR and TMPRSS2 in LNCaP analyzed by Western blot analysis (n = 2). g Expression of AKT1 analyzed by quantitative analysis after 22RV1 cells were treated with indicated compounds. *P < 0.05 versus DMSO group (n = 5 in each group).