Fig. 7. Harmine has no effect on apoptosis and cell viability of hESC-derived cardiomyocytes.

a Representative flow cytometric analyses of apoptosis in hESC-derived cardiomyocytes following treatment of harmine (25 μM) and NE (20 μM). Cells were double labeled with Annexin V-FITC and propidum iodide after harvesting. The upper-right quadrant represents the annexin V⁺/PI⁺ cells; the lower-right quadrant represents the annexin V⁺/PI⁻ cells; the upper-left quadrant represents the annexin V⁻/PI⁺ cells. b–d Quantification of the ratio of annexin V⁺/PI⁻cells, annexin V⁻/PI⁺ cells and annexin V⁺/PI⁺ cells. e Dose–response curves demonstrating the effect of harmine on cell viability of hESC-derived cardiomyocytes using CellTiter-Glo Assay (n = 4 conducted per concentration). Data are mean ± SEM of three independent experiments.