Fig. 1. Effect of wogonin on HG-treated MPC5 cells’ viability and inhibition on HG-induced inflammation.

a The molecular structural formula of wogonin. b MTT assay to determine the effect of wogonin on viability of MPC5 cells and HG-treated MPC5 cells. c Western blot of WT-1, nephrin, and podocin in MPC5 cells. d Real-time PCR analysis of TNF-α, MCP-1, and IL-1β expression in MPC5 cells. e Western blot analysis of phosphorylated p65 (p-p65) in MPC5 cells. Results represent mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs NC. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs HG. HG high glucose, MG 5.5 mM glucose plus 24.5 mM mannitol, NC normal control, Wog wogonin