Fig. 6. Bcl-2 overexpression and treatment with wogonin have similar cellular protective roles.

a Real-time PCR analysis of Bcl-2 in MPC5 cells. b Western blot analysis of Bcl-2 in MPC5 cells. c Western blot analysis of WT-1 and nephrin in MPC5 cells. d Western blot analysis of cleaved caspase-3, Beclin-1, ATG7, and p62 in MPC5 cells. Results represent means ± SEM for three independent experiments. **P < 0.01, ***P < 0.001 vs NC. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs HG. ^$$P < 0.01, ^$$$P < 0.001 vs Bcl-2-OE group. HG high glucose, Wog wogonin, EV empty vector, OE overexpression, NC normal control