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. 2021 Sep 3;43(1):76–85. doi: 10.1038/s41401-021-00764-8

Fig. 3. 7w and 7x inhibited LPS-induced inflammatory cytokine expression in macrophages.

Fig. 3

J774a.1 macrophages were pretreated with 7w or 7x at 1.25, 2.5, 5, or 10 μM for 30 min and then treated with LPS at 0.5 μg/mL for 24 h. DMSO was used as vehicle control. a TNF-α and b IL-6 levels in the medium were determined by ELISA. Data were normalized to total protein concentration from the same plate. For the detection of mRNA levels, MPMs were pretreated with vehicle control (DMSO), 7w (5 or 10 μM) or 7x (5 or 10 μM) for 30 min followed by incubation with LPS at 0.5 μg/ml for 6 h. c, d The mRNA levels of inflammatory cytokines TNF-α, IL-6, VCAM-1, and ICAM-1 were determined by RT-qPCR and normalized by β-actin mRNA level. Data were mean ± SEM of 3–5 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with LPS group.