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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biochimie. 2021 Sep 25;192:51–62. doi: 10.1016/j.biochi.2021.09.013

Fig. 10.

Fig. 10.

Time-dependent [2Fe-2S] cluster transfer from holo SyNfu to apo human GLRX2 (A) and apo S. cerevisiae Grx3 (C) recorded by CD in 50 mM HEPES, 100 mM NaCl, pH 7.5 at a 1:1 concentration ratio. Spectra were recorded every 2 min (A, B, and C) or 10 sec (D) after the addition of holo SyNfu, and converted to percent cluster transfer, (B) or (D), to calculate the second-order rate constant based on the concentration of the [2Fe-2S] cluster. The rate constants for of cluster transfer to apo GLRX2 and Grx3 were calculated to be 1220 M−1min−1 and 21821 M−1min−1, respectively. For both (A) and (C), the starting time (tinitial) is denoted by the (black) trace, and the tfinal is denoted by the (purple) trace.