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Extraction of the [2Fe-2S] cluster from reconstituted holo SyNfu to form the [2Fe-2S](GS)4 complex monitored by UV-Vis. a) Time course measurement showing a decrease in the characteristic Fe-S cluster bands at 420 and 330 nm. b) Change in absorbance for SyNfu in the absence (black) and presence (red) of 1 mM GSH. c) Linear dependence of observed rate constant with GSH concentration.
