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. 2021 Dec 21;9:751785. doi: 10.3389/fcell.2021.751785

FIGURE 3.

FIGURE 3

mTORC1 signaling regulates LL37-induced cutaneous angiogenesis in vivo. (A,B) Immunostaining of pS6 and CD31 (A), and quantitative analysis of pS6 and CD31 double positive cells (indicated by white arrows) (B) in skin tissues from healthy skin and rosacea patients. (C,D) Immunostaining of pS6 and CD31, and quantitation of pS6 and CD31 double positive cells (indicated by white arrows) in mice skin tissues treated with LL37 and/or RAPA. (E,F) Immunostaining of CD31, and quantitative analysis of the number of CD31+ vessels in control and LL37-treated rosacea-like mouse model treated with or without RAPA. (G,H) Immunostaining of CD31, and quantitation of CD31+ vessels in WT and TSC2+/− mouse skin treated with or without LL37. (I) Vegf mRNA expression in WT and TSC2+/− mouse skin treated with or without LL37 by RT-qPCR. (A,C,E) Right panels indicate the magnified picture in the left box with the same color. Scale bar: 50 μm. Data represent mean ± SEM from three independent experiments. Two-tailed unpaired Student’s t-test (B) or 1-way ANOVA with Bonferroni’s post hoc test (D,F,H,I) was used *p < 0.05 and **p < 0.01.