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. 2021 Dec 21;9:751785. doi: 10.3389/fcell.2021.751785

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Copyright © 2021 Peng, Sha, Liu, Chen, Xu, Xie, Deng and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mTORC1 signaling regulates the tube formation mediated by LL37 in HUVECs. (A) HUVECs tube formation assay in presence of LL37. VEGF-treated group was set as a positive control. (B) Quantitative analysis of the tubular structure in (A). (C) pS6 and S6 protein levels in LL37-treated HUVECs by western blotting. Tubulin was taken as a loading control. pS6 protein level was analyzed relative to total S6. (D) pS6 and S6 protein levels in LL37 and/or RAPA-treated HUVECs. Tubulin was taken as a loading control. pS6 protein level was analyzed relative to total S6. (E) HUVECs tube formation assay treated with LL37 and/or RAPA. (F) Quantitative analysis of the tubular structure in (E). Scale bar: 50 μm. Data represent mean ± SEM from three independent experiments. Statistical significance was determined by One-way ANOVA (B,F). *p < 0.05 and **p < 0.01.