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. 2021 Dec 21;12:796590. doi: 10.3389/fphar.2021.796590

FIGURE 4.

FIGURE 4

DG ameliorated intestinal barrier impairment and low-grade inflammationin of colon in high fat diet mice. (A) H&E staining, PAS staining for goblet cells, and Immunohistochemistry staining for MUC2 (scale bar: 50 μm). (B) The number of positive cells of PAS and MUC2 staining in each colonic crypt (n = 5). (C) Relative mRNA expression of colonic occludin and MUC2 (n = 8). (D) Relative mRNA expression of colonic IL-1β and IL-6 (n = 8). (E) Immunofluorescence staining for ZO-1 distribution of colon tissues (red, scale bar: 50 μm). Values were denoted as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Abbreviations: DG, diammonium glycyrrhizinate. MUC2, mucin 2. ZO-1, zonula occludens 1.