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. 2021 Dec 21;12:796590. doi: 10.3389/fphar.2021.796590

TABLE 1.

Primers sequences of target genes used in real-time PCR.

Primers Sequence
mGAPDH Forward 5′ GGA​GAA​ACC​TGC​CAA​GTA​TG 3′
Reverse 5′ TGG​GAG​TTG​CTG​TTG​AAG​TC 3′
mIL-1β Forward 5′ GTG​GCT​GTG​GAG​AAG​CTG​TG 3′
Reverse 5′ GAA​GGT​CCA​CGG​GAA​AGA​CAC 3′
mIL-6 Forward 5′ CCA​GTT​GCC​TTC​TTG​GGA​CT 3′
Reverse 5′ GGT​CTG​TTG​GGA​GTG​GTA​TCC 3′
mTNF-α Forward 5′ CTT​CTG​TCT​ACT​GAA​CTT​CGG​G 3′
Reverse 5′ CAG​GCT​TGT​CAC​TCG​AAT​TTT​G 3′
mOccludin Forward 5′ CGG​TAC​AGC​AGC​AAT​GGT​AA 3′
Reverse 5′ CTC​CCC​ACC​TGT​CGT​GTA​GT 3′
mOccludin Forward 5′ TCG​CCC​AAG​TCG​ACA​CTC​A 3′
Reverse 5′ GCA​AAT​AGC​CAT​AGT​ACA​GTT​ACA​CAG​C 3′
mZO-1 Forward 5′ GGG​CCA​TCT​CAA​CTC​CTG​TA 3′
Reverse 5′ AGA​AGG​GCT​GAC​GGG​TAA​AT 3′
mFXR Forward 5′ GGA​CGG​GAT​GAG​TGT​GAA​G 3′
Reverse 5′ TGA​ACT​TGA​GGA​AAC​GGG​AC 3′
mCYP7A1 Forward 5′ AGG​CAT​TTG​GAC​ACA​GAA​G 3′
Reverse 5′ACA​GAT​TGG​AGG​TTT​TGC​AT 3′