FIG. 1.

Cloning of the e(y)2 gene. (A) Map of the obtained clone showing site of the Stalker insertion. Black boxes indicate the regions of localization of corresponding transcripts. Arrows show the direction of transcription. B, BamHI; X, XhoI. The underlined fragment was used for the rescue of the wild phenotype. (B) Northern blot hybridization with poly(A)⁺ RNA from males of Oregon R and e(y)1¹ strains. XhoI-BamHI fragment was used as a probe. (C) Schematic presentation of the construct used for phenotype rescue. Shaded boxes indicate polylinker pCaSpeR 3 vector, and transcripts are indicated by black arrows showing the direction of the transcription. The inserted genomic region includes the whole e(y)2 transcript (0.5 kb) with the adjacent 5′ sequences and the 3′ portion of the 2.2-kb neighboring transcript. The two 3′ ends of the transcripts slightly overlap.