FIG. 2.

e(y)2 homologues from different species. (A) Result of sequence alignment of e(y)2 from different species. Identical amino acids are represented in dark boxes; similar ones are represented by light boxes. Ho, Homo sapiens; Xe, Xenopus laevis; Zb, Danio rerio (zebrafish); Ha, Halocynthia roretzi; St, Strongylocentrotus purpuratus; Ze, Zea mays; Gl, Glycine max; Sc, Schistosoma mansoni; Dr, Drosophila melanogaster; Di, Dictyostelium discoideum; Br, Brugia malayi. (B) Antibodies against Drosophila e(y)2 recognize human homologue. The immunoprecipitation with the antibodies against e(y)2 and preimmune serum (PI) of nuclear extracts of HeLa and Schneider (Sch) cells are shown. We used SDS–15% PAGE for resolution of the proteins. A Western blot was probed with the antibodies against Drosophila e(y)2. (C) Map of the human e(y)2 gene obtained from the sequence of chromosome 8 (accession number AC021237). Short segments indicate the positions of individual CpG sites.