FIG. 5.

e(y)2 is associated with chromatin. (A) Immunostaining of polytene chromosomes from Oregon R larvae with antibodies to e(y)2 and Cy3-conjugated secondary antibodies. Magnification, ×1,000. (B) Control immunostaining with preimmune serum and Cy3-conjugated secondary antibodies. (C) Western blot of cytoplasmic (CE) and nuclear (NE) extracts from Drosophila embryos probed with antibodies to e(y)2. (D) Binding of e(y)2 to chromatin immobilized on paramagnetic beads. Incubation of beads (B), chromatin (B+Ch), or naked DNA (B+DNA) with nuclear extract (NE), is as indicated above each lane.