Fig. 3.

Dynamic N-glycoproteomic analysis integrated with proteomic quantification during L6 myotube formation. Quantification of glycopeptides plotted against changes in their total protein levels at day 1 (A) and day 7 (B) of differentiation relative to day 0. The green box highlights glycopeptides from integrin α-3 (ITGA3). The arrow indicates annotated HCD-MS/MS spectrum in panel C identifying a glycopeptide containing Asn605 in the N-glycosylation motif (NxS/T/C) with the glycan HexNAc(4)Hex(6)Fuc(1) corresponding to a glycan composition with biantennary terminal di-Gal. D, Relative changes of the indicated glycopeptide and the total protein levels of ITGA3 across the differentiation time course. HCD, higher collisional dissociation; MS/MS, tandem mass spectrometry.