A summary overview of peptides identified across the different analyses. A summary overview of the number of peptides identified by matching the DIA of RA957, JY, and OD5P immunopeptidomics data against each of the sample-specific, combined, mixed, and BigLib libraries (A). A summary overview of the fraction of peptides predicted as binders in RA957 DIA samples matched against the RA957-specific, RA957+JY, OD5P+RA957, mixed, and BigLib libraries applying q values of 0.01 and against the BigLib library applying q values of 0.05, 0.1, 0.15, and 0.2, respectively (B). The total number of identified peptides in each of the abovementioned assays is reported. DIA, data-independent acquisition.