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. 2021 Mar 12;22(5):e51412. doi: 10.15252/embr.202051412

Figure 3. ER protein reflux is constitutive in cancer cells.

Figure 3

  • A–C
    Subcellular protein fractionation of several ER‐resident proteins in (A) GL261, (B) U87 and (C) A549 cells treated with the indicated concentrations of Tm or Tg using Digitonin. (NP40 represents the membrane fraction extracted with NP40 Cell Lysis Buffer)
  • D–F
    Quantification of the subcellular protein fractionation of several ER endogenous proteins in GL261, U87, and A549 cells as in (A–C), respectively. Biological triplicates, mean ± SD calculated using Prism 9 (GraphPad).
  • G
    Subcellular protein fractionation of several ER‐resident proteins in A549 cells treated with the indicated concentrations of Tm or Tg using differential centrifugation protocol.
  • H
    Quantification of the subcellular protein fractionation of several ER endogenous proteins in A549 as in (G). Biological triplicates, mean ± SD calculated using Prism 9 (GraphPad).