Skip to main content
. 2021 Nov 15;41(1):e107640. doi: 10.15252/embj.2021107640

Figure 5. Direct interaction between SRSF1 and U1 snRNP.

Figure 5

  1. Overlay of the 2D 13C‐1H HMQC of 13C ILV‐labelled SRSF1∆RS before and after addition of 1 molar equivalent of in vitro reconstituted U1 snRNP.
  2. Overlay of the 2D 13C‐1H HMQC of 13C ILV‐labelled SRSF1∆RS before and after addition of 1 molar equivalent of the ssRNA target of SRSF1.
  3. Overlay of the 2D 13C‐1H HMQC of 13C ILV‐labelled SRSF1ΔRS before and after addition of 1 molar equivalent of U1 snRNA stem‐loop 3.
  4. Schematic representation of SRSF1ΔRS bound on U1 snRNP.
  5. Bar plot showing the chemical shift perturbations of the SRSF1 methyl groups when the protein was titrated with U1 snRNP, ssRNA or U1 snRNA SL3.