Figure EV3. Effects of selected compounds on scrambled‐GP and ‐GA levels.

• A, B
  Lysates from NSC34 cells transfected with scrambled‐GPx100 and treated for 24 h with DMSO, GELD, SPL, FSK, H89. (A) Immunoblot for poly‐GP expression. Tubulin was used as a loading control. Quantification from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, *P < 0.05; **P < 0.01; ***P < 0.001. (B) Quantification of PBS‐insoluble poly‐GP (FRA). Quantification from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, **P < 0.01; ***P < 0.001.
• C, D
  Lysates from NSC34 cells transfected with scrambled‐GAx100 and treated for 24 h with DMSO, GELD, SPL, FSK, H89. (C) Immunoblot for poly‐GA expression. Tubulin was used as a loading control. Quantification from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, **P < 0.01; ***P < 0.001. (D) Quantification of PBS‐insoluble poly‐GA (FRA) from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, *P < 0.05; **P < 0.01; ***P < 0.001.
• E
  Lysates from NSC34 cells transfected with scrambled‐GPx100, treated for 24 h with GELD, SPL and/or 16 h with 10 µM MG132. Quantification of PBS‐insoluble poly‐GP (FRA) from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, *P < 0.05; ****P < 0.0001.
• F
  Lysates from NSC34 cells transfected with scrambled‐GPx100, treated for 24 h with SPL and/or 10 mM 3MA. Quantification of PBS‐insoluble poly‐GP (FRA) from three biological replicates. Data are mean ± SEM. One‐way ANOVA followed by Uncorrected Fisher's LSD, ***P < 0.001.

Source data are available online for this figure.