Figure 6. H89 and PKA knockdown improve motility defects in a Drosophila model of C9ALS/FTD.

• A, B
  Representative graphs showing the climbing ability flies carrying the UAS‐(G4C2)x36 construct in neurons using the Elav‐gal4 promoter upon treatment with H89 10 µM diluted with 0.1% of DMSO and 5% sucrose in PBS. The wild‐type (w¹¹¹⁸ ) line was used as a control. (A) Female flies (♀). Data are mean ± SD. Two‐way ANOVA followed by Tukey's multiple comparisons, significance is reported for the 6^th day of treatment ****P < 0.0001 (G4C2)x36 versus (G4C2)x36 + H89. (B) Male flies (♂). Data are mean ± SD. Two‐way ANOVA followed by Tukey's multiple comparisons, significance is reported for the 6^th day of treatment. ****P < 0.0001 (G4C2)x36 versus (G4C2)x36 + H89. Experiments were performed three times, N = 15 adult flies were used for each genotype, sex and treatment (N tot = 45).
• C, D
  Representative graphs showing climbing activity of flies co‐expressing the UAS‐Pka‐RNAi construct (1, v330111) in combination with UAS‐(G4C2)x36 in neurons using the Elav‐gal4 promoter. The wild‐type (w¹¹¹⁸ ) line was used as a control. (C) Female flies (♀). Data are mean ± SD. Two‐way ANOVA followed by Tukey's multiple comparisons, significance is reported for the 9^th day ****P < 0.0001 (G4C2)x36 versus Pka‐RNAi (1);(G4C2)x36. (D) Male flies (♂). Data are mean ± SD. Two‐way ANOVA followed by Tukey's multiple comparisons, significance is reported for the 7^th day ****P < 0.0001 (G4C2)x36 versus Pka‐RNAi (1);(G4C2)x36. Experiments were performed twice with different RNA interfering sequence, N = 15 adult flies were used for each genotype and sex (N tot = 30).