Fig. 3.

Effects of LCH on human skin cancer cell cycle distribution. (A, B) A375 and A431 cells were treated with 0, 10, 20, and 30 μM LCH for 48 h, stained with PI, and analyzed for DNA content by FACS analysis. The data represent mean percentages ± SD (n=3; **p<0.01, ***p<0.001 compared to the control group). (C) A375 and A431 cells treated with 0, 10, 20, and 30 μM LCH for 48 h were harvested to prepare cell extracts. The cell extracts were subjected to SDS-PAGE and Western blot analysis to detect the levels of p53, p27, p21, and cyclin-D1. β-Actin was used as the loading control.