Fig. 4.

Elution of permethylated glycans expressed in terms of glucose units. A, reduced and ¹³C-permethylated Dextrans (isomaltooligosaccharide series, DP4 to 10) were separated using a PepMap Acclaim C18 column in 1 mM lithium acetate-containing mobile phases (45–70% B over 150 min). Extracted ion chromatogram for each predicted mass is shown (within 10 ppm). Retention times of DP standards, run both before and after sample acquisition queue, were averaged and plotted using a linear regression fitting. Error bars represent standard deviation (See inset). B, reduced and ¹³C-permethylated CFG N-Glycan standards (107–110, 121) were analyzed as described in (A). C, summary table of analyzed CFG standards with retention times converted to reversed phase glucose units (RP g.u.) using linear regression fitting shown in A, inset. CFG, Consortium for Functional Glycomics; DP, degree of polymeriation.