Fig. 7.
Analysis of reduced and permethylated O-glycans released from mouse brain proteins and human cell lines defective in the O-mannosylation pathway.A, extracted ion chromatogram of Hex1HexNAc1 structures released from mouse brain proteins indicates two structural isomers. MS2 fragmentation of m/z 518.3 at the indicated retention times are shown as insets. B, LC-MS analysis of O-glycans released from HEK293 WT and ΔPOMGNT1 mutant cell lines. Respective EICs and annotated MS2 spectra are shown. EICs, extracted ion chromatograms.