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. 2021 Mar 20;20:100074. doi: 10.1016/j.mcpro.2021.100074

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 1 — General CS/DS structure and our employed workflow for structural domain mapping of PG-derived GAGs.A, structure and domain distribution of a typical PG-derived CS/DS GAG. B, schematic view of GAGDoMa sample preparation and analysis. C and D, rationale for including benzonase and hyaluronidase digestions in the sample preparation. Total ion chromatograms before and after benzonase digestion (C) display interference of oligonucleotides and indicate incomplete GAG depolymerization with chondroitinase ABC without benzonase digestion. Extracted ion chromatograms (XICs) at m/z 757.22 before and after hyaluronidase digestion (D) display a reduction in HA variants upon digestion. The peak indicated by an asterisk arises from background noise.