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. 2021 Mar 20;20:100074. doi: 10.1016/j.mcpro.2021.100074

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — CS/DS linkage regions from INS-1832/13 cells. A, heatmap summary of the linkage region variants generated after chondroitinase ABC (ChABC; gray) depolymerization (top) and an illustration displaying observed modifications and peptide variants (bottom). B and C, HCD-MS² spectra of linkage region hexasaccharides at m/z 588.1340 (2–) corresponding to ΔL6S1Ser (S) (B), and at m/z 628.1121 (2–) corresponding to ΔL6S2S (C), both at NCE 70%. D, heatmap summary of the linkage region variants generated after chondroitinase AC (ChAC; orange) and chondroitinase B (ChB; blue) depolymerizations. The two most common structures after each depolymerization are displayed in the boxes (ChAC, orange; ChB, blue) underneath the heatmap. E, HCD-MS² spectrum at m/z 516.1523 (2–) at NCE 60% corresponding to the main linkage region variant generated after chondroitinase AC depolymerization, ΔL4S0Ser-Gly-Glu-Glu (EEGS). F, HCD-MS² spectrum at m/z 850.8217 (3–) at NCE 60% corresponding to the main linkage region variant generated after chondroitinase B depolymerization, ΔL12S4S. Mass accuracies of precursor and fragment ions are found in supplemental Tables S1 and S2.