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. 2021 Mar 20;20:100074. doi: 10.1016/j.mcpro.2021.100074

Fig. 5.

Fig. 5

Intact CS GAGs from INS-1832/13 cells.A, MS1 spectrum at 52.18 min displays variants of intact CS L19S8 precursor ions and charge state distribution thereof. The inset shows the isotopic pattern of the [M+3DBA-7H]4– precursor ion at m/z 1154.2881 and the monoisotopic mass accuracy (Δm –2.3 ppm) from the theoretical mass. B, HCD-MS2 spectrum at m/z 1154.2891 (4–) where important fragment ions are annotated to confirm the assignment in (A). C, heatmap summary of intact CS GAGs after heparinase (Hep; purple) and chondroitinase B (ChB; blue) depolymerizations. D, total intensity comparison of intact GAGs obtained after heparinase (Hep; purple) and chondroitinase B (ChB; blue) depolymerizations (mean ± SD). Mass accuracies of fragment ions are found in supplemental Table S2. DBA, dibutylamine.