FIG. 2.

ACLP immunostaining on 15.5-dpc mouse embryo. Wild-type 15.5-dpc embryos were fixed in methyl Carnoy's fixative, dehydrated, and embedded in paraffin. Sagittal sections (5 μm) were incubated with a polyclonal ACLP antiserum (A and C to H) or preimmune serum (B) and detected as described in Materials and Methods. (A) ACLP expression in 15.5-dpc embryo. Original magnification, ×10. (B) Preimmune control staining. Original magnification, ×10. (C) Expression in large blood vessels (lumen indicated by ∗) and ribs (arrowheads). Original magnification, ×100. (D) Higher magnification of panel C showing expression surrounding the smooth muscle cells (arrowhead) of a large blood vessel. Original magnification, ×200. (E) Expression in periosteum of rib (arrowhead). Original magnification, ×400. (F and G) Expression in basement membrane of lung (arrowhead). Original magnification, ×400. (H) Dermal layer of skin (arrowhead). Original magnification, ×400.