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Top, DiPyrO labeling of glycosylamine; Red dots represent heavy isotopic atoms (15N418O) in the light DiPyrO tag; blue dots represent heavy isotopic atoms (2H6) in the heavy DiPyrO tag.Bottom, workflow for the relative quantification of DiPyrO-labeled N-glycans illustrating the microenvironment. Adapted from Chen et al. (66) with permission.
