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Left, structure and isotope configurations of four-plex SUGAR tags.Purple dot: 13C, orange dot: 2H, red dot: 15N. Right, ESI-MS/MS fragmentation comparison of aminoxyTMT-labeled and SUGAR-labeled N-glycans. AminoxyTMT-labeled H8N2 ([aminoxyTMT − H8N2 + 2H]2+) at NCE 25 (A) and 30 (C), SUGAR-labeled H8N2 ([SUGAR − H8N2 + 2H]2+) at NCE 25 (B) and 30 (D). Adapted from Feng et al. (68) with permission.
