A, the major biosynthetic steps and enzymes involved in core1 to 4 O-glycan structures with extensions and capping by sialic acid are illustrated.B, overview of Glyco-DIA libraries. The Glyco-DIA library consists of several sublibraries, including Tn-DIA libraries from SC cell lines, T-DIA libraries from WT cell lines, T-DIA library from human blood serum, and in silico expanded libraries. LFQ, label-free quantification. Reprinted from Ye et al. (183) with permission from the author.